COVID-19 Testing Method Developed by IIT Delhi Approved

COVID-19 Testing Method Developed by IIT Delhi Approved
Image : IIT Delhi

Developed by the Indian Institute of Technology (IIT), a method to detect COVID-19, making it affordable for a large population in the country, which will make it affordable, has been approved by the ICMR, officials said on Thursday. IIT Delhi is the first educational institution to receive ICMR approval for a real-time PCR-based diagnostic assay. The development also comes against the backdrop of the Indian Council of Medical Research (ICMR) as testing has been halted for COVID-19 cases due to huge variation in test results, as test results vary drastically, further halting investigations. Is complicated for.

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Officials said that the current testing methods are “investigation-based”, while a “probe-free” method developed by the IIT team, which reduces test costs without compromising accuracy.

The test method has been approved by the ICMR. The assay has been validated in ICMR with sensitivity and specificity of 100 percent. This IITD is the first educational institution to receive ICMR approval for real-time PCR-based diagnostic assay, ”a senior official told PTI.

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“This is the first test-free assay for ICMR-approved COVID-19 and would be useful for specific and inexpensive high throughput testing. This assay can be easily extended as it does not require a fluorescent probe. The official said that the team is aiming for large-scale deployment of the kit at an affordable cost with suitable industrial partners at the earliest.

RNA or ribonucleic acid is one of the major biological macromolecules required for all known forms of life. It plays various important biological roles related to protein synthesis such as transcription, decoding, regulation and expression of genes.

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“Using comparative sequence analysis, we have identified unique regions in COVID-19. These unique regions are not particularly present in other human coroners providing the opportunity to detect COVID-19, ”Professor Vivekanandan Perumal, a key member of the team told PTI.

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The primer set, targeting unique regions in the spike protein of COVID-19, was designed and tested using real-time polymerase chain reaction. The group-designed primers specifically bind to conserved regions in more than 200 fully sequenced COVID-19 genomes. The sensitivity of the in-house assay is compared to commercially available kits, ”he said.

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